ChIP-seq
Brief

Chromatin Immunoprecipition (ChIP) is a classical experimental method to study the interaction between protein and DNA. The combination of ChIP and high-throughput sequencing (ChIP Sequencing) can screen and identify protein binding sites in the whole genome, and is widely used in the research of histone modification, transcription factor regulation and other related fields.

Fields
  • Gene expression regulation
  • Epigenetic heterogeneity
  • Embryonic development
  • Disease research
  • Genetic imprinting
Process
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Parameters
Product TypeSequencing StrategyRecommended Data AmountCycle
ChIP-seq

NovaSeq 6000 PE150

DNBSEQ-T7 PE150

≥ 20 M reads40 days

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Requirements
Product TypeSampling Requirements
ChIP-seqSample type: chlP enriched DNA and corresponding input samples
Sample demand: ≥5 ng;
Sample concentration: ≥0.4 ng/μL;
Sample purity: OD260/280=1.8-2.2
DNA fragment size: The main belt of electric ice ranges from 150 bp to 500bp, the main peak ranges from 200 bp to 300bp, and the main bands are clear;
Please provide the detection result.
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