10x Genomics is a single-cell library preparation system based on microfluidic technology, which can simultaneously obtain more than 1,000-80,000 single cells, prepare libraries compatible with Illumina platform, classify cell populations and analyze differences in gene expression between cell populations through sequencing of high-throughput single cell data. 10x Genomics single-cell expression analysis is an excellent method for studying tumor heterogeneity, immune cell population, and embryonic development with a wide range of cell types including tumor cells, immune cells, stem cells, nerve cells, germ cells, and embryonic cells.