Third-generation Animal and Plant Resequencing

Brief

The third-generation genome resequencing of animal and plant is a genome sequencing based on next-generation sequencing technology, which mainly focuses on the analysis of single nucleotide variation (SNP) and small fragment insertion deletion mutation (InDel), and faces challenges in the detection of variation in some repeated regions and structural variation in large fragments (SV). PacBio long-read sequencing can effectively cover long structural variation sequences, and low-depth third-generation sequencing can accurately identify most structural variations in animal and plant genomes.

Parameters
Type of Variant DetectionLibrary Type
SNP、InDel、SV15k /20 k HiFi DNA library
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Requirements
Library TypeSampling Requirements

HiFi DNA Library

Total DNA: HiFi≥6 μg

Concentration: ≥30 ng/μL

Quality: genome integrity, DNA main band: ≥30kb

Purity: OD260/280=1.8-2.0; OD260/230=1.6-2.5; NC/QC=0.5-2

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