Brief
Single cell RNA sequencing is a high-end technology for whole-transcriptome amplification and high-throughput sequencing of mRNA at the single-cell level, which studies the overall gene expression in a single cell, as well as the structural variation of genes; it is mainly used to study cell heterogeneity in cellular and molecular mechanisms, and the sample size is too small for conventional high-throughput sequencing.
Requirements
| Sample Requirements | |
| Cell Isolation | The single cell isolation is completed by the partner. |
| Sample Type | Fresh intact cells in vivo; samples not suitable for fixation, staining, etc. |
| Sample Volume | The volume of single cell samples separated by microscope or other equipment should not be higher than 1 μL. |
| Sample Quantity | It is recommended that the number of repeated single cell samples be no less than 5. |
| Other Requirements | 0.2 mL PCR tubes with flat cover provided by the company are directly used for collection, and their covers are numbered; Cell samples should be washed with buffer solution; Repeated freeze-thaw and foaming of the lysate should be avoided. |
| During the operation, please try to place the sampling tube in a low temperature environment (e.g., ice). The sample collection tubes should be wrapped and sealed with parafilms, put into a PCR tube box, fixed with rubber bands, then temporarily stored at 80℃ in ziplocked bags, and transported with large-volume dry ice; Cells should be prevented from being attached to the upper inner wall of the sample tube which is caused by being inverted in liquid state. | |
| Sample volume: for single-cell transcriptome amplification, it is recommended that the number of repeated single-cell samples be no less than 5; | |
| After sampling, cells should be placed in 96-well tube boxes and sent as soon as possible on the premise of avoiding dumping of sample tubes. If necessary, please store them at -80℃. |
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