10x Genomics Single Cell Immune Profiling

Brief

Annoroad Genome's 10x Genomics automated cell sorting platform based on microfluidic technology enables reverse transcription and amplification of single cell 5' transcripts, and simultaneous preparation of single cell 5' transcriptome library and immune library V(D)J library. The 10x Genomics platform enables automatic sorting of up to 80,000 cells in 7 minutes. Each GEM contains a single cell and reverse transcription reaction system, which can simultaneously obtain single cell 5 'gene expression data and TCR/BCR V(D)J rearrangement information, in-depth study of the complexity of cellular immune response, so that it has a wide application prospect in immunology and immuno-oncology.

     
Requirements

Sampling Requirements
Sample TypeFresh Samples, Cryopreserved Samples, Fresh Biofluids, Cell&Nuclear Suspension; Samples not suitable for fixation, staining, etc.
Total Amount of SampleTotal amount >6*105 PCS; Duplicate aliquots from one cell sample are processed in parallel: for QC and experimental use.
Sample Concentration700-1200 cells/μL
Sample QualityCells should be free of clumps, with less fragments, survival rate >90%, cell diameter< 30 μm, and free of reverse transcription inhibitors and non-cellular nucleic acid molecules
Cell Cryoprotectant SamplePlease consult local dealers for details
Slide left and right to see more》